Enzyme Degassing for Oxygen-Sensitive Reactions in Open Vessels of an Automated Parallel Synthesizer RAFT Polymerizations

ACS Publications

An enzyme degassing method for oxygen-intolerant polymerizations was implemented in a commercially available automated parallel synthesizer and tested for reversible addition–fragmentation chain transfer (RAFT) polymerizations performed in open vessels. For this purpose, a recently reported methodology that employs the enzyme glucose oxidase (GOx) to deplete oxygen in reaction media was utilized. The effectiveness of this approach to perform unattended parallel polymerization reactions in open vessels was demonstrated by comparing experimental results to those obtained under similar experimental conditions but utilizing the common degassing method of sparging N2 to remove oxygen. The proposed experimental technique displayed good precision in performing RAFT polymerizations and good control of the obtained polymers and could be easily adapted to other systems where the removal of oxygen is mandatory. This alternative high-throughput/high-output method may have the potential to increase productivity in research projects where oxygen-intolerant reactions are involved.

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Enzyme Degassing for Oxygen-Sensitive Reactions in Open Vessels of an Automated Parallel Synthesizer RAFT Polymerizations

Mu Wang a,c, Junliang Zhang b,c, Carlos Guerrero-Sanchez c,d, Ulrich S. Schubert c,d, Anchao Feng a and San H. Thang a,e

a Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Materials Science and Engineering, Beijing University of Chemical  Technology, Beijing 100029, P. R. China

b MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions, Shaanxi Key Laboratory of Macromolecular Science and Technology, Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, P. R. China

c Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany

d Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany

e School of Chemistry, Monash University, Clayton Campus, VIC 3800 Australia

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ACS Publications
https://doi.org/10.1021/acscombsci.9b00082
Copyright © 2019 American Chemical Society

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April 30, 2020